Proteoglycan compositions for the treatment of cardiovascular inflammatory diseases

ABSTRACT

Compositions with synergistic anti-inflammatory effects in inflammatory diseases resulting from activation and consequent degranulation of mast cells and followed by secretion of inflammatory biomolecules from the activated mast cells, composed of a heavily sulfated, non-bovine proteoglycan such as shark cartilage chondroitin sulfate C, and one or more of a hexosamine sulfate such as D-glucosamine sulfate, a flavone such as quercetin, an unrefined kernel olive oil that increases absorption of these compositions in various routes of administration, S-adenosylmethionine, a histamine-1 receptor antagonist, a histamine-3 receptor agonist, an antagonist of the actions of CRH, caffeine, and a polyamine.

[0001] This application is a divisional/CIP of copending PCT/US02/00470, filed Jan. 3, 2002, which is a CIP of copending U.S. Pat. No. 09/771669, filed Jan. 30, 2001m which is a divisional of U.S. Pat. No. 09/056707, filed Apr. 8, 1998.

BACKGROUND OF THE INVENTION

[0002] The invention is generally related to the treatment of inflammatory conditions. More specifically, the invention is related to compositions containing inhibitors of mast cell activation and secretion such as a proteoglycan that are designed to be used as dietary supplements or adjuvants to conventional approved medications for the relief of cardiovascular inflammatory conditions.

[0003] There have been a number of mostly anecdotal reports that the proteoglycan chondroitin sulfate, as well as glucosamine sulfate, a product of the intestinal breakdown of proteoglycans, may be helpful in relieving the pain of osteoarthritis: —Shute N. Aching for an arthritis cure. US News and World Report, Feb. 10, 1997.—Cowley G. The arthritis cure? Newsweek, Feb. 17, 1997; Foreman J., People, and their pets, tout arthritis remedy. The Boston Globe, Apr. 7, 1997; Tye L. Treatment gains scientific attention. The Boston Globe, Sep. 25, 2000.

[0004] A recent meta-analysis showed potential therapeutic benefit of chondroitin sulfate and/or glucosamine in osteoarthritis [McAlindon et al. J Am Med Assn. 283:1469 (2000)], while a double-blind clinical trial with glucosamine showed definite benefits in osteoarthritis with respect to both pain and radiographic joint appearance [Reginster et al., Lancet 337:252 (2001)]. However, I ss than 5% of the chondroitin sulfate in commercially available preparations is absorbed orally, because the size of the molecule and the degree of sulfation impede its absorption from the gastrointestinal tract. Furthermore, such commercial preparations use chondroitin sulfate obtained from cow trachea, with the possible danger of contracting spongiform encephalopathy or “mad cow disease”. In fact, the European Union has banned even cosmetics that contain bovine-derived products.

[0005] Theoharides et al. British Journal of Pharmacology 131:1039 (2000) indicated for the first time how proteoglycans such as chondroitin sulfate may work. The paper reported that chondroitin sulfate and, to a lesser degree, glucosamine sulfate, inhibit activation of mast cells that are known to trigger allergy and asthma. This discovery is the basis for Theoharides, U.S. patent applications Ser. Nos. 09/056,707, filed Apr. 8, 1998 and 09/773,576, filed Feb. 2, 2001.

[0006] Mast cells are also now recognized as important causative intermediary in many painful inflammatory conditions[Galli, N Eng J Med. 328:257 (1993); Theoharides, Int J Tissue Reactions 18:1 (1996)], such as interstitial cystitis and irritable bowel syndrome [Theoharides, Ann NY Acad, Sci. 840:619 (1998)], as well as in migraines and possibly multiple sclerosis [Theoharides, Persp Biol Med. 26:672 (1983); Theoharides, Life Sci 46:607 (1996)]. In fact, glucosamine was recently considered to be prophylactic for migraines [Russell, Med Hypoth 55:195 (2000)].

[0007] Mast cells are increasingly implicated in conditions involving inflamed joints, such as in osteoarthritis and rheumatoid arthritis, through activation of local mast cells by, for example, neuropeptides, such as Substance P. Additional indirect evidence also supports the involvement of mast cells in bone resorption: (a) systemic mastocytosis is invariably associated with osteoporosis; (b) inhibition of mast cell mediator release reversed lytic bone changes; (c) depletion of mast cells inhibited bone resorption in organ culture; (d) human synovial mast cells were shown to secrete in response to allergic and non-immunologic stirnili, (e)-human mast cells release the cytokine IL-6 and (f) IL-6 has been definitively linked to bone resorption and osteoporosis.

[0008] It was recently shown that chondroitin sulfate's ability to inhibit the activation of mast cells compliments the inhibitory effects on mast cell activation of another class of naturally occurring compounds, the flavonoids [Middleton et al. Pharm Rev 52:1 (2000)]. Certain plant flavones (in citrus fruit pulp, seeds, sea weed) are now recognized as anti-allergic, anti-inflammatory, anti-oxidant and cytoprotective with possible anti-cancer properties. Only some flavonoids that belong to the subclass of flavones, e.g., quercetin, inhibit mast cell activation.

[0009] Quercetin inhibits secretion from human activated mast cells [Kimata et al. Allergy 30:501(2000)], and has also been used effectively for the treatment of chronic prostatitis [Shoskes et al., Urology 54:960 (1999)]. However, other flavonoids may have opposite effects. Use of the term “bioflavonoids” or “citrus flavonoids” in certain commercial products, therefore, provides little information, and may include molecules that have detrimental effects; for example, soy contains isoflavones that have estrogen-like activity that worsens inflammatory conditions.

[0010] Copending U.S. patent applications Ser. Nos. 09/056,707, 09/771,669, 09/773,576, and 10/439,301 claim the oral and topical use of proteoglycans, without and with flavonoids, for the treatment of mast cell activation-induced diseases. Absorption of these compositions from the gastrointestinal tract and synergism with other treatment modalities were addressed in these applications.

[0011] Applicant has described the use of antagonists of the action of Corticotropin Releasing Hormone (also known as Corticotropin Releasing Factor) in inhibiting myocardial mast call activation in myocardial ischemia (copending U.S. patent application Ser. No. 08/858,136, filed May 18, 1997), in treating stress induced skin disease (U.S. Pat. No. 6,020,305) and stress induced migraine headaches (U.S. Pat. No. 5,855,884), the contents of which are incorporated herein by reference.

[0012] Histamine-3 receptor agonists as pharmaceutical agents in mast cell-involved diseases are described in Theoharides U.S. Pat. No. 5,831,259. The contents of these three patents are incorporated herein by reference. At the time of this invention the synergistic effects of the present compositions with such antagonists had not yet been recognized.

[0013] An important need therefore exists for compositions for administration to human patients being treated for mast cell-induced cardiovascular inflammatory diseases by various modalities, that are synergistic in that they have stronger effects than the sum of the effects of the individual components, and also synergistic with conventional clinical treatments of inflammatory conditions. “Synergistic” is also intended to mean: “coordinated or correlated action by two or more structures or drugs” [Stedman's Medical Dictionary, 23rd edition, Williams & Wilkins, Baltimore, 1976]. An important need also exists for formulations that increase the absorption from the gastrointestinal tract, nasal passages and skin surface of the compositions of the invention. Such formulations have been discovered, and are described below.

SUMMARY OF THE INVENTION

[0014] The invention comprises compositions for human use containing a sulfated proteoglycan and an unrefined olive kernel (pit) oil (hereinafter referred to as olive kernel extract (“OKE”), and one or more active ingredients selected from the group consisting of a sulfated hexosamine, a flavonoid compound, S-adenosylmethionine (“SAM”), histamine-1 receptor antagonists, histamine-3 receptor agonists, antagonists of the actions of CRH, caffeine, folic acid, rutin, bitter willow bark extract, hyaluronate salts such as sodium hyaluronate, polyunsaturated fatty acids such as eicosapentenoic acids, niacin, polyamines, a chemoth rapeutic agent such as tamoxifen, an interferon, together with appropriate excipients and carriers, said compositions having improved absorption from the gastrointestinal tract, skin surface, and nasal and pulmonary surfaces, and anti-inflammatory effects synergistic with each other and synergistic with available conventional clinical treatment modalities.

[0015] In one embodiment, the sulfated glucosamine is D-glucosamine sulfate, the proteoglycan is non-bovine chondroitin sulfate, and the flavone is quercetin.

[0016] In an other embodiment, compositions may also contain antagonists of the effects of CRH on mast cells or other target cells of the myocardium, gastric mucosa, urinary bladder, skin, meningeal membranes, and blood-brain barrier.

[0017] In still another embodiment, the present compositions are used against superficial vasodilator flush syndromes.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] It has been discovered that a combination of a sulfated proteoglycan and a unique, unrefined olive kernel extract, together with one or more of a sulfated D-hexoseamine, a flavone, an CRH antagonist, a histamine-1 receptor antagonist, a histamine-3 receptor agonist, a rutin, a polyunsaturated fatty acid such as eicosapentenoic acid, niacin, polyamines, caffeine, a chemotherapeutic agent such as tamoxifen, an interferon, has synergistic anti-inflammatory effects when used as a dietary supplement, a topical product or an aerosol for nasal or pulmonary adminstration, without or with a conventional clinical treatment for inflammatory diseases. Such inflammatory diseases result from the activation, degranulation and consequent secretion of vasoactive and inflammatory biochemicals from mast cells, and the resultant inflammatory diseases include the group consisting of: allergic inflammation, arthritis (to include osteoarthritis and rheumatoid arthritis), cancer, cardiovascular diseases, fibromyalgia, inflammatory bowel disease, interstitial cystitis, irritable bowel syndrome, migraines, angina, chronic prostatitis, eczema, multiple sclerosis, psoriasis, sun burn, periodontal disease of the gums, superficial vasodilator (flush} syndromes due, for example, to niacin ingestion, and hormonally-dependent cancers.

[0019] Other natural sulfated proteoglycans suitable for practicing this invention include keratan sulfate, dermatan sulfate and hyaluronic acid salts, e.g., sodium hyaluronate. The preferred biological source of the chondroitin sulfate is shark cartilage which is more-highly sulfated than the common commercial chondroitin sulfate isolated from cow trachea; the shark cartilage source also avoids the potential dangers associated with bovine sources.

[0020] The highly preferred flavone is quercetin which inhibits secretion of inflammatory molecules from mast cells by affecting moesin, a unique 78 kDa mast cell protein [Theoharides et al. J Pharm Exp Therap 294:810 (2000)]. In addition to quercetin, other flavones suitable in carrying out the invention include myricetin, genistein and kaempferol.

[0021] It should be emphasized that the olive kernel oil, which expression is used interchangeably herein with the expression olive kernel extract, is not olive oil per se. Olive oil, which has as its source the flesh of the olive, is discarded in step (1) of the procedure described below. The material used in this invention is an extract derived from the kernel (pit) of the olive.

[0022] The olive kernel oil component of the inventive compositions is preferably an unrefined (first pressing, filtered, oleic acid-related acidity <1%, water content <5%) extract produced, for one source, on the island of Crete in Greece. This kernel oil is especially prepared by the maker by a process consisting essentially of: (1) washing the kernel mass that remains after the compression of the oil from the olive flesh with water; (2) drying the washed kernels in a stream of hot air; (3) extracting the dried kernels with hexane and steam; (4) microfiltering the extract (1 micron pore size) to remove particulate matter; (5) heating the extract at 86-100 degrees while percolating helium (to avoid oxidation) through the fluid to evaporate the hexane (final <1%), which process reduces the water content to <1% and the acidity as oleic acid of <3%.

[0023] This olive kernel extract surprisingly has the unique property of increasing absorption of the other components of the anti-inflammatory compositions through the intestinal mucosa or skin (cf., Example 17 below), and also adds its own content of important anti-oxidants [Bosku, World Rev Nutr Diet, 87:56 (2000)], such as omega fatty acids (e.g., eicosapentenoic acid) and alpha tocopherol. Although not claimed herein, it has been claimed that kernel olive extract has cytoprotective, longevity-producing effects [Trichopoulou et al. Am J Clin Nutr 61:1 346S (1995); Trichopoulou et al, Cancer Epid Biomarker Prevention 9:869 (2000)]. The polyphenols in such kernel extract also have anti-inflammatory effects in, for example, arthritis [Martinez-Dominguez etal., Inflamm. Res. 50:102 (2001)]. A preferred source of the unrefined kernel olive extract of the invention is: E.B.E.K., Inc., Commercial, Industrial Enterprises of Crete, 118 Ethnikis Antistasecos, Heraklion, Crete, 71306, Greece.

[0024] Supplementation of the compositions described above with the methylation reagent S-adenosylmethionine (“SAM”) adds antioxidant, anti-inflammatory and cytoprotective properties, particularly in inflammatory joint diseases. Addition of SAM also accelerates metabolism of homocysteine, which amino acid has been implicated in coronary disease, to cysteine, which is harmless. Folic acid may be added to certain of the present formulations for similar reasons.

[0025] In a highly preferred embodiment, the sulfated proteoglycan is non-bovine chondroitin sulfate, preferably from shark cartilage, which blocks mast cell activation, degranulation and consequent secretion of inflammatory biochemicals from the mast cells. As noted above, other natural sulfated proteoglycans suitable for practicing this invention include keratan sulfate, dermatan sulfate and hyaluronic acid salt, e.g., sodium hyaluronate.

[0026] The highly preferred flavone is quercetin which inhibits secretion of inflammatory molecules from mast cells by affecting moesin, a unique 78 kDa mast cell protein [Theoharides et al. J Pharm Exp Therap 294:810 (2000)]. In addition to quercetin, other flavones suitable in carrying out the invention include myricetin, genistein and kaempferol. One preferred source of quercetin is the Saphora plant or rutin (quercetin glycoside).

[0027] Another supplement to the basic compositions of the invention is a histamine-1 receptor antagonist, such as diphenhydramine, hydroxyzine, azelastine, azatadine and cyproheptadine. Other histamine-1 receptor antagonists are described in Table 25-1 in Goodman and Gilman's The Pharmaceutical Basis of Therapeutics, 9^(th) ed., New York, 1996. Histamine -3 receptor agonists are described in the Theoharides patents listed above.

[0028] Inhibitors of mast cell activation and secretion may be used in the treatment of inflammatory processes such as superficial vasodilator syndrome, e.g., menopausal-associated flush, monosodium glutamate-associated flush, carcinoid flush and niacin-associated flush.

[0029] Sources of CRH antagonists include, in addition to the Theoharides patents listed in the Background section above: Neurocrine Biochem. Inc.'s D—Phe 12 Nle Ala32,21,38hCRH(12-41)NH2, cat no. 1P-36-41; Pfizer non-peptide CP-154,526-1; Sigma Chem., St. Louis anti-CRH polyclonal antiserum; and Pfizer, NY patents and applications: U.S. Pat. Nos. 6,211,195, 5,795,905, PCT/IB95/00573, PCT/IB95/00439, U.S. Pat. Nos. 08/448,539, 08/481,413, 09/735,841, and in Owens t al. Pharm. R v. 43-425 (1991).

[0030] The preferred concentration range of the proteoglycan, hexosamine sulfate and flavone components of the oral formulations are 10-3,000 mg per tablet or capsule. The preferred concentration range for SAM is 3-1,000 mg per capsule or tablet. The preferred concentration range of bitter willow extract is 10-50 mg per tablet or capsule. Generally, where present, the amounts of the olive kernel extract are at least three times those of the other active ingredients, preferably 900-1200 mg. Polyunsaturated fatty acids, preferably from fish oils, e.g., eicosapentenoic acid, may be also present, preferably in amounts similar to that of the oliver kernet extract, e.g., 100-300 mg per capsule or tablet. The number of capsules or tablets to be taken per day is determined by the nature and severity of the medical condition, and is readily determinable by the patient's health care provider. Other representative formulations are described in the examples below.

[0031] The compositions of the invention may be formulated in any standard means of introducing pharmaceuticals into a patient, e.g., by means of tablets or capsules. The compositions of the invention include ointments and creams for skin conditions, mouth washes and toothpaste for periodontal diseases, and solutions for nasal aerosols. Standard excipients and carriers, e.g., beeswax and lecithin of about 25-100 mg per unit dose, for the active ingredients of the inventive compositions are described in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa.

[0032] Although not bound by any particular mechanism of action of the components of the claimed compositions, the inventor contemplates that the proteoglycan may inhibit the activation and degranulation of the relevant mast cells, while the flavone may inhibit the secretion of inflammatory biomolecules from these mast cells. “Activation” and “degranulation” of mast cells are defined herein as is standard and well known in this art, that is, to mean secretion from the activated mast cell of any type of molecule(s) that alone or in combination triggers inflammatory processes.

EXAMPLES Example 1

[0033] Table 1 compares chondroitin sulfate-containing commercial products to the present compositions. TABLE 1 Comparison of commercial chondroitin sulfate-containing products to present embodiments Product Available compositions Present invention Main ingredient Mixture of bovine Non-bovine chondroitin chondroitins sulfate, preferably the C type Source Cow trachea Shark cartilege Amount/cap or tab. 100-300 mg 10-3000 mg Degree of sulfation Zero to low High Target Unspecified Mast cells, inflammatory cells Other ingredients Vitamins, fish oils Flavones, unrefined kernel olive oil, SAM, histamine-1 receptor antagonists, histamine-3 receptor agonists, CRH antagonists, polyamines, caffeine, folic acid Advantages None known Anti-allergic, anti- inflammatory, anti- oxidant, cytoprotective Adverse effects Risk of mad cow None known disease, spongiform encephalopathy, stomach upset, allergy to fish products Relevant Osteoarthritis Allergic inflammation conditions angina, asthma coronary artery disease, arthritis (osteoarthritis or rheumatoid arthritis), chronic prostatitis, eczema, fibromyalgia, interstitial cystitis, irritable bowel syndrome, inflammatory bowel disease, migraines, multiple sclerosis, psoriasis, periodontal disease, flush syndrome, cancer (including hormonally-dependent forms). Scientific None found Theoharides et at. Br J publications Pharm 131:1039 (2000) Middleton et al. Pharm Rev 52:673 (2000)

[0034] In all examples that follow, chondroitin sulfate is of a non-bovine variety.

Example 2

[0035] Composition For Protecting Against Inflammatory Diseases)

[0036] Two capsules to be taken orally 2-3 times daily, at least one hour before meals Ingredients, per capsule, mg: *Chondroitin sulfate 150-300 *OKE  900-1200 *D-Glucosamine sulfate 150-300 *Quercetin 150-300

Example 3

[0037] Composition For Protecting Against Arthritis Ingredients per capsule, mg: *OKE  900-1200 *D-Glucosamine sulfate 150-300 *Chondroitin sulfate 150-300 *A hyaluronate salt 100-200 *Quercetin 150-300

Example 4

[0038] Topical Composition For Protecting Against Arthritis

[0039] Skin ointment or cream. Apply three times per day to affected areas. Ingredients % by weight *OKE 15  *D-glucosamine sulfate 5 *Chondroitin sulfate 5 *A hyaluronate salt 5 *Bitter willow bark extract 5 *Quercetin 3

Example 5

[0040] Composition For Protecting Against Cardiovascular Disease

[0041] Two capsules to be taken orally 2-3 times per day, in mg: *OKE  900-1200 *Chondroitin sulfate  50 *Kaempferol 100 *S-adenosylmethionine  50 *Niacin 100 *Bitter willow bark extract, 5% by weight

Example 6

[0042] Composition For Protecting Against Periodontal Disease Mouthwash: *Chondroitin sulfate 0.4 M *Quercetin 0.4 M *In a standard mouthwash vehicle

Example 7

[0043] Toothpaste Composition Toothpaste, mg %: *Chondroitin sulfate 5 *Quercetin 3 *Optionally, D-glucosamine sulfate 5 *In a standard toothpaste vehicle

Example 8

[0044] Sunscreen Composition Ingredients mg % *Chondroitin sulfate 5 *D-glucosamine sulfate 5 *Quercetin 3 *Sun screen (e.g., TiO₂) 5

Example 9

[0045] Composition For Protecting Against Migraine Headaches Ingredients, mg: *Chondroitin sulfate  50 *Quercetin 100 *Azatadine  4 *Optionally, a CRH-receptor antagonist

Example 10

[0046] Composition For Protecting Against Relapsing Multiple Sclerosis Ingredients, mg: *Chondroitin sulfate  50 *Quercetin 400 *Hydroxyzine  50 *Optionally, int rferon-beta

Example 11

[0047] Composition For Protecting Against Cystitis And Prostatitis Ingredients, mg: *OKE  900-1200 *D-glucosamine sulfate  50 *Chondroitin sulfate 100-300 *Sodium hyaluronate 200 *Quercetin 100-400

Example 12

[0048] Composition For Protecting Against “Flush” Ingredients, per capsule: *Chondroitin sulfate 50 mg *Quercetin 150 mg *Bitter willow bark extract 5% by weight *Optionally, cyproheptadine or azatadine 4 mg

Example 13

[0049] Cream Composition For Protecting Against Skin Allergy Ingredients: % by weight *OKE 15  *Aloe vera 5 *Non-bovine chondroitin sulfate 5 *Myricetin 5 *Alpha-tocopherol  I 5 *Optionally, azelastine or hydroxyzine 5

Example 14

[0050] Composition For Protecting Against Allergy and Allergic Asthma Ingredients, mg Myricetin 500 Chondroitin sulfate 200 Optionally, azelastine or hydroxyzine

Example 15

[0051] Composition For Protecting Against Hormonally-Dependent Cancers Ingredients, mg Quercetin 150  Genestein 50 Optionally, tomoxifen or raloxifen 10

Example 16

[0052] Composition For Protecting Against Allergic Conjunctivitis Ingredients: *Quercetin 0.05% *Chondroitin sulfate 2.0% *Optionally, azelastine 0.05%

FIG. 17

[0053] Composition for Protection Against Cardiovascular Inflammatory Disease Ingredients, mg per capsule Chondroitin sulfate 25-75 OKE 200-300 Quercetin  50-150 Rutin 25-75 SAM 150-250 Folic acid 0.17-0.37 Fish oil polyunsaturated 150-200 fatty acids Bitter willow extract 10-50 Suspension agents 50-70

Example 18

[0054] Effect of Olive Kernel Extract on Absorption of Proteoglycan Sulfate In Vivo

[0055] Chondroitin sulfate was tritiated by New England Nuclear Corp. to a specific activity of 4.3 mCi/ml.

[0056] 2.5 mCi of tritiated chondroitin sulfate was given orally to 250 g laboratory rats without (control) and with (experimental) OKE. Serum radioactivity was measured 8 hours thereafter.

[0057] The results showed that, in control animals, about 3.9% of the dose reached the circulation. In sharp contrast, in animals given OKE along with the labeled chondroitin sulfate, about 14.3% of the dose was absorbed into the general circulation.

[0058] These results demonstrate that OKE increased by almost 400% the absorption of a proteoglycan from the intestine into the general circulation. 

I claim:
 1. A composition with synergistic anti-inflammatory properties in conditions induced by the activation of mast cells, consequent degranulation of said cells and secretion of inflammatory biomolecules, comprising a non-bovine proteoglycan sulfate and an unrefined olive kernel extract (“OKE”), and one or more of a hexosamine sulfate, a flavone, S-adenosylmethionine (“SAM”), a histamine-1 receptor antagonist, a histamine-3 agonist, an antagonist of the actions of Corticotropin Releasing Hormone (“CRH”), a hyaluronate salt, a rutin, a niacin, a polyunsaturated fatty acid, a polyamine, caffeine and an anti-cancer chemotherapeutic agent, in an appropriate excipient or vehicle.
 2. The composition according to claim 1, wherein said sulfated proteoglycan is selected from the group consisting of non-bovine chondroitin sulfate, keratan sulfate, dermatan sulfate and a hyaluronate salt.
 3. The composition according to claim 2, wherein said chondroitin sulfate is chondroitin sulfate C derived from shark cartilage.
 4. The composition according to claim 1, wherein said hexosamine sulfate is D-glucosamine sulfate.
 5. The composition according to claim 1, wherein said flavone is selected from the group consisting of quercetin, myricetin, genistein, rutin and kaempferol.
 6. The composition according to claim 1, wherein said unrefined-kernel extract contains polyphenols and alpha-tocopherol.
 7. The composition according to claim 1, said composition being for oral use, comprising 10-3,000 mg per capsule or tablet of each of non-bovine chondroitin sulfate C, quercetin and D-glucosamine sulfate, with 900-1200 mg of OKE.
 8. The composition according to claim 7, further supplemented with 3-1,000 mg of SAM per capsule or tablet.
 9. A composition according to claim 1, wherein said inflammatory diseases are selected from the group consisting of: arthritis, cancers, fibromyalgia, inflammatory bowel disease, interstitial cystitis, irritable bowel syndrome, migraines, angina, chronic prostatitis, eczema, multiple sclerosis, psoriasis, sun burn, tooth decay, periodontal disease, stressed-induced migraines, stress-induced opening of bladder mucosa, stress-induced opening of the blood-brain barrier, superficial vasodilator (flush) syndrome, and hormonally-dependent cancers.
 10. The composition according to claim 9, wherein said inflammatory disease is arthritis and said composition is for oral administration, comprising non-bovine chondroitin sulfate, OKE, quercetin, D-glucosamine sulfate, and, optionally, sodium hyaluronate.
 11. The composition according to claim 9, wherein said inflammatory disease is arthritis and said composition is for topical use, comprising, non-bovine chondroitin sulfate, OKE, D-glucosamine sulfate, quercetin, sodium hyaluronate, and bitter willow bark extract.
 12. The composition according to claim 9 for oral or aerosol use in allergic conditions, comprising non-bovine chondroitin sulfate, OKE and a flavonoid selected from the group consisting of quercetin, myricetin and kaempferol, and, optionally, a histamine-1 receptor antagonist.
 13. The composition according to claim 9, for topical use in allergic conditions, comprising non-bovine chondroitin sulfate, OKE, myricetin, alpha-tocopherol, and, optionally, a histamine-1-receptor antagonist.
 14. The composition according to claim 13, wherein said antagonist is diphenhydramine, hydroxyzine, azatadine, azelastine or cyproheptadine.
 15. The composition according to claim 9 wherein said inflammatory disease is superficial vasodilator “flush” syndrome, said composition comprising a non-bovine proteoglycan, OKE, a flavonoid, bitter willow bark extract, and, optionally, cyproheptadine or azatadine.
 16. The composition according to claim 9, wherein said inflammatory disease is multiple sclerosis, said composition comprising non-bovine chondroitin sulfate, OKE, quercetin or myricetin, hydroxyzine, and, optionally, caffeine, SAM and interferon-beta.
 17. The composition according to claim 9, wherein said inflammatory disease is migraine headaches, and said composition comprises non-bovine chondroitin sulfate, OKE, quercetin, and azatadine.
 18. The composition according to claim 1, said composition being for oral use, comprising 150-300 mg per capsule or tablet of each of non-bovine chondroitin sulfate, quercetin and D-glucosamine sulfate, with 900-1200 mg of OKE, and, optionally, 100-200 mg sodium hyaluronate and/or 100 mg SAM.
 19. The composition according to claim 1, said composition consisting of an ointment or cream for topical application, comprising, in % by weight, non-bovine chondroitin sulfate, 5; OKE, 15; D-glucosamine sulfate, 5; quercetin, 3; sodium hyaluronate 5; and, bitter willow bark extract
 5. 20. The composition according to claim 19 supplemented by at least one of the following, each 1-5 mg %: histamine-1 receptor antagonists diphenhydramine, hydroxyzine, azelastine, azatadine and cyproheptadine.
 21. The composition according to claim 1, said composition compromising a mouth wash composition, consisting of non-bovine chondroitin sulfate and quercetin, each 0.3-0.4 M; OKE, 1% (v/v); and, optionally, at least one of D-glucosamine sulfate, 0.4 M and SAM, 0.15 M, in a mouth wash vehicle.
 22. The composition according to claim 1, said composition consisting of a tooth paste, comprising, in mg%, non-bovine chondroitin sulfate, 5; OKE,1; quercetin, 3, and, optionally, D-glucosamine sulfate, 5, in a tooth paste vehicle.
 23. The composition according to claim 1, said composition consisting of a sunscreen composition, comprising, in mg%, non-bovine chondroitin sulfate, 5; OKE, 10; quercetin 3; and at least one of D-glucosamine sulfate, 5, and titaniun dioxide, 5, in a sun screen vehicle.
 24. The composition according to claim 1, for use in treating migraine headaches, said composition comprising, in mg, non-bovine chondroitin sulfate, 50; OKE, 150; guercetin, 100; azatadine, 4; and, optionally, a CRH antagonist.
 25. The composition according to claim 1, said composition comprising, in mg, non-bovine chondroitin sulfate, 50: quercetin, 400; hydroxyzine, 50; and, optionally, a CRH antagonist.
 26. The composition according to claim 1, said composition comprising, in mg, non-bovine chondroitin sulfate, 100; OKE, 900-1200; D-glucosamine sulfate, 50; and quercetin,
 100. 27. The composition according to claim 1, comprising, in mg %, non-bovine chondroitin sulfate,-5-OKE, 900-1200, D-glucosamine sulfate, 5; and quercetin,
 3. 28. The composition according to claim 1, wherein said inflammatory disease is cancer and wherein said composition is designed for oral use, comprising 25-50 mg of genistein and 150-300 mg of quercetin, and 900-1200 mg OKE.
 29. The composition according to claim 1, wherein said inflammatory disease is atherosclerosis with or without myocardial ischemia, comprising 100-300 mg each of non-bovine chondroitin sulfate, myricetin, folic acid and SAM, and 900-1200 mg OKE, in a vehicle for oral use.
 30. The composition according to claim 1, wherein said inflammatory disease is interstitial cystitis or prostatitis, said composition comprising, in mg, 100-300 of non-bovine chondroitin sulfate, 900-1200 of OKE, 50-300 D-glucosamine sulfate, 100-300 of sodium hyaluronate, 100-400 quercetin, in a vehicle for oral use.
 31. The composition according to claim 1, wherein said inflammatory disease is multiple sclerosis, said composition comprising, in mg, 50-300 each of non-bovine chondroitin sulfate, myricetin, hydroxyzine and SAM, 900-1200 of OKE, and, optionally, interferon-beta, in a vehicle for oral use.
 32. The composition according to claim 1, said composition comprising, in mg, non-bovine chondroitin sulfate 200; OKE, 450; myricetine, 200; and diphenhydramine, 5 mg.
 33. The composition according to claim 1, said composition comprising, in mg, non-bovine chondroitin sulfate, 50; OKE, 900-1200; kaempferol, 100; SAM, 50; folic acid, 50; and niacin,
 100. 34. The composition according to claim 1, wherein said inflammatory disease is superficial vasodilation flush syndrome, said composition comprising 50 mg non-bovine chondroitin sulfate; OKE, 450 mg; 350 mg quercetin, 5% by weight bitter willow bark extract, and, optionally, 4 mg cyproheptadine or azatadine.
 35. The composition according to claim 1, wherein said inflammatory disease is skin allergy, said composition comprising, in % by weight, 5 each of aloe vera, non-bovine chondroitin sulfate and alpha-tocopherol, 15 of OKE, and, optionally, azelastine.
 36. The composition according to claim 1, wherein said inflammatory disease in allergy or allergic asthma, comprising 200 mg of myricetin, 200 mg of non-bovine chondroitin sulfate, and, optionally, azelastine or hydroxyzine.
 38. The composition according to claim 1, wherein said inflammatory disease is a hormonally-dependent cancer, comprising, in mg, non-bovine chondroitin sulfate, 150; OKE, 450; quercetin, 250; genistein, 50; and, optionally, tamoxifen or raloxifen,
 10. 39. The composition according to claim 1, wherein said inflammatory disease is allergic conjunctivitis, comprising quercetin, 0.05%; non-bovine chondroitin sulfate, 2.0%; OKE, 0.001%; and, optionally, azelastine 0.05%. 